Tissue-engineering repair of extrahepatic bile ducts

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• 作者：Alejandro José ; Pé ; rez Alonso ; MD ; PhD^a ; ^{apma85@hotmail.com} ; Carlos Del Olmo Rivas ; MD^b ; Ignacio Machado Romero ; MD^b ; Francisco Javier Cañ ; izares Garcia ; MD ; PhD^c ; Pablo Torne Poyatos ; MD ; PhD^b
• 关键词：Bile duct ; Tissue engineering ; Transplant ; Repair
• 刊名：Journal of Surgical Research
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：179
• 期：1
• 页码：18-21
• 全文大小：551 K

文摘

Background

In recent years, with the widespread use of laparoscopic cholecystectomy and liver transplantation, complications involving the biliary system are increasing. All techniques available have a high risk of recurrence or high-morbidity problems. We developed a three-dimensional collagen duct modified with agarose hydrogel, to substitute the affected extrahepatic bile duct.

Materials and methods

We used it in 40 guinea pigs and studied the histology and physiology at 4 wk, 3, and 6 mo after transplantation. Blood test, histologic techniques, and cholangiography were performed in all of them.

Results

All experimental animals survived up to their sacrifices. Our graft showed highly potential applications to treat hepatobiliary diseases that require surgery.