Semen characteristics and refrigeration in free-ranging giant anteaters (Myrmecophaga tridactyla)

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• 作者：Camila do Nascimento Luba^a ; Yatta Linhares Boakari^a ; Alexandre Martins Costa Lopes^b ; Marcelo da Silva Gomes^b ; Flá ; via Regina Miranda^b ; Frederico Ozanan Papa^a ; Joã ; o Carlos Pinheiro Ferreira^a ; ^{jcferreira@fmvz.unesp.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：In situ ; Electroejaculation ; Reproduction ; Xenarthra ; Pilosa ; Myrmecophagidae
• 刊名：Theriogenology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：84
• 期：9
• 页码：1572-1580
• 全文大小：1456 K

文摘

The giant anteater (Myrmecophaga tridactyla) is considered vulnerable to extinction. Scientific data on the reproductive parameters of this species are scarce. Semen from eight free-ranging giant anteaters was collected to establish its characteristics and the effects of cooling and storage at 5 °C after dilution with the BotuCrio extender without cryoprotectant. The ejaculate presented two distinct sequential fractions, including a whitish fraction, which was milky and rich in sperm cells, and a gel fraction, which was colorless, viscous, and azoospermic. The mean ± standard error of the mean values of the seminal characteristics were as follows: volume of the first fraction, 0.75 ± 0.1 mL; motility, 75 ± 2.9%; vigor, 3.2 ± 0.3; sperm motility index, 68.8 ± 4.3; concentration, 108.5 ± 13.4 × 10⁶/mL; plasma membrane integrity index, 71 ± 4.0%; spermatic defects detected using modified Karras staining, 35.5 ± 3.3%; and spermatic alterations identified by differential interference contrast microscopy, 48.3 ± 6.8%. During refrigeration, the semen presented decreasing motility from 0 to 18 hours, sperm motility index decreased from 0 to 24 hours, and vigor did not change in the first 6 hours and then decreased to 18 hours.