Pharmacological characterization of the inhibition by moxonidine and agmatine on the cardioaccelerator sympathetic outflow in pithed rats

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• 作者：Luis E. Cobos-Puc ; Carlos M. Villal&#xf3 ; n ; Martha B. Ramí ; rez-Rosas ; Araceli Sá ; nchez-L&#xf3 ; pez ; Jair Lozano-Cuenca ; Benjamí ; n G&#xf3 ; mez-Dí ; az ; Antoinette MaassenVanDenBrink ; David
• 关键词：α ; ₂-adrenoceptor ; AGN192403 ; BU224 ; Imidazoline receptors ; Moxonidine ; Sympatho-inhibition
• 刊名：European Journal of Pharmacology
• 出版年：2009
• 出版时间：15 August 2009
• 年：2009
• 卷：616
• 期：1-3
• 页码：175-182
• 全文大小：838 K

文摘

This study analysed the inhibition produced by the agonists moxonidine (imidazoline I₁ receptors > α₂-adrenoceptors) and agmatine (endogenous ligand of imidazoline I₁/I₂ receptors), using B-HT 933 (6-ethyl-5,6,7,8-tetrahydro-4H-oxazolo[4,5-d]azepin-2-amine dihydrochloride; α₂-adrenoceptors) for comparison, on the rat cardioaccelerator sympathetic outflow. Male Wistar rats were pithed and prepared to stimulate the cardiac sympathetic outflow or to receive i.v. bolus of exogenous noradrenaline. Sympathetic stimulation or noradrenaline produced, respectively, frequency-dependent and dose-dependent tachycardic responses. I.v. continuous infusions of moxonidine (3 and 10 µg/kg min), agmatine (1000 and 3000 µg/kg min) and B-HT 933 (30 and 100 µg/kg min) inhibited the tachycardic responses to sympathetic stimulation, but not those to noradrenaline. The cardiac sympatho-inhibition by either moxonidine (3 µg/kg min) or B-HT 933 (30 µg/kg min) was not modified by i.v. injections of saline or the antagonists AGN192403 [(±)-2-endo-Amino-3-exo-isopropylbicyclo[2.2.1]heptane hydrochloride; 3000 µg/kg; imidazoline I₁ receptors] or BU224 (2-(4,5-dihydroimidazol-2-yl)quinoline hydrochloride; 300 µg/kg; imidazoline I₂ receptors) and abolished by rauwolscine (300 µg/kg; α₂-adrenoceptors). At the same doses of these compounds, the sympatho-inhibition to moxonidine (10 µg/kg min) and agmatine (1000 µg/kg min) was: (1) not modified by saline, AGN192403 or BU224; (2) partially blocked by rauwolscine or the combination of rauwolscine plus BU224; and (3) abolished by the combination of rauwolscine plus AGN192403. These results demonstrate that the cardiac sympatho-inhibition to: (1) 3 µg/kg min moxonidine or 30 µg/kg min B-HT 933 involves α₂-adrenoceptors; and (2) 10 µg/kg min moxonidine or 1000 µg/kg min agmatine involves α₂-adrenoceptors and imidazoline I₁ receptors.