Enhancement of antibody and cellular immune responses to malaria DNA vaccines by in vivo electroporation
详细信息 查看全文
- 作者：Carlota Dobañ ; o ; Georg Widera ; Dietmar Rabussay ; Denise L. Doolan
- 关键词：Malaria ; DNA vaccine ; Electroporation
- 刊名：Vaccine
- 出版年：2007
- 出版时间：4 September 2007
- 年：2007
- 卷：25
- 期：36
- 页码：6635-6645
- 全文大小：709 K
文摘
We evaluated the effectiveness of in vivo electroporation (EP) for the enhancement of immune responses induced by DNA plasmids encoding the pre-erythrocytic Plasmodium yoelii antigens PyCSP and PyHEP17 administered intramuscularly and intradermally to mice. EP resulted in a 16- and 2-fold enhancement of antibody responses to PyCSP and PyHEP17, respectively. Immunization with 5 μg of DNA via EP was equivalent to 50 μg of DNA via conventional needle, thus reducing by 10-fold the required dose to produce a given effect. Moreover, IFN-γ responses were increased by approximately 2-fold. Data demonstrate the potential of EP to enhance immune responses to DNA vaccines against infectious agents.