Iron overload enhances human mesenchymal stromal cell growth and hampers matrix calcification
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- 作者：Adriana Borrielloa ; adriana.borriello@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Ilaria Caldarellia ; Maria Carmela Speranzaa ; Saverio Scianguettab ; Annunziata Tramontanoa ; Debora Bencivengaa ; Emanuela Stamponea ; Aide Negric ; Bruno Nobilib ; Franco Locatellid ; Silverio Perrottab ; Adriana Olivaa ; Fulvio Della Ragionea ; fulvio.dellaragione@unina2.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：hBM-MSCs ; Human bone marrow mesenchymal stromal cells ; DCF-DA ; 2&prime ; 7&prime ; -Dichlorofluorescein diacetate ; ROS ; Reactive oxygen species ; FAC ; Ferric ammonium citrate ; SAC ; Sodium ammonium citrate ; SM ; Standard medium ; OM ; Osteogenic medium ; AP ; Alkaline phosphatase ; PNPP ; para-nitrophenyl phosphate ; OC ; Osteocalcin ; Tf ; Transferrin ; TfR ; Transferrin receptor ; ARS ; Alizarin red staining ; qPCR ; Quantitative PCR
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：1860
- 期：6
- 页码：1211-1223
- 全文大小：2979 K
文摘
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Bone marrow mesenchymal stromal cells (BM-MSCs) are the osteoblasts precursors and key hematopoietic niche components.
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Iron enters BM-MSCs through transferrin-dependent and -independent mechanisms.
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Iron, at concentrations detected in sera of iron overloaded patients, stimulates in vitro the proliferation of BM-MSCs.
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Iron-dependent growth stimulation is associated with cyclins E and A increase, p27 decrease and MAPK pathway activation.
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Iron impairs BM-MSCs osteoblastic differentiation acting both on cell commitment and matrix calcification process.