Scaling-up batch conditions for efficient sucrose hydrolysis catalyzed by an immobilized recombinant Pichia pastoris cells in a stirrer tank reactor

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• 作者：Duniesky Martí ; nez^a ; Carmen Mené ; ndez^b ; L&aacute ; zaro Hern&aacute ; ndez^b ; Alina Sobrino^a ; Luis E. Trujillo^b ; ^c ; ^{letrujillo3@espe.edu.ec} ; Ivan Rodrí ; guez^d ; Enrique R. Pé ; rez^a ; ^{enrique.perez@cigb.edu.cu}
• 关键词：Bioreactor ; Calcium alginate beads ; Enzymatic biocatalyst ; Immobilized biocatalyst ; Industrial biotechnology ; Invert sugar ; Invertase ; Kinetic model ; Scale-up ; Stirred tank reactor ; Sucrose inversion
• 刊名：Electronic Journal of Biotechnology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：25
• 期：Complete
• 页码：39-42
• 全文大小：220 K
• 卷排序：25

文摘

Invert sugar is used greatly in food and pharmaceutical industries. This paper describes scaling-up batch conditions for sucrose inversion catalyzed by the recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase entrapped in calcium alginate beads. For the first time, we describe the application of a kinetic model to predict the fractional conversion expected during sucrose hydrolysis reaction in both, a model and a prototype bioreactor with 0.5- and 5-L working volume, respectively.ResultsDifferent scaled-up criteria used to operate the 0.5-L bioreactor were analyzed to explore the invert sugar large scale production. After model inversion studies, a 5-L scaled-up reaction system was performed in a 7-L stirred reactor. Both scaled-up criteria, immobilized biocatalyst dosage and stirring speed, were analyzed in each type of bioreactors and the collected data were used to ensure an efficient scale-up of this biocatalyst.ConclusionsTo date, there is not enough information to describe the large-scale production of invert sugar using different scaled-up criteria such as dose of immobilized biocatalyst and stirring speed effect on mass transfer. The present study results constitute a valuable tool to successfully carry out this type of high-scale operation for industrial purposes.