Role of CrAT in macrophage inflammation was tested in vivo. Myeloid CrAT does not regulate inflammation during HFD-induced obesity. Fasting-induced metabolic stress is not regulated by myeloid CrAT expression. CrAT expression in myeloid cells does not regulate LPS-induced acute endotoxemia. Mitochondrial acetyl-CoA efflux via CrAT does not regulate macrophage inflammation.