Examination of real-time PCR for HIV-1 RNA and DNA quantitation in patients infected with HIV-1 BF intersubtype recombinant variants
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- 作者：N. Schvachsa ; G. Turk ; M. Burgard ; D. Dilernia ; M. Carobene ; M. Pippo ; M. Gó ; mez-Carrillo ; C. Rouzioux ; H. Salomon
- 关键词：HIV-1 viral load ; Real-time PCR ; bDNA
- 刊名：Journal of Virological Methods
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：140
- 期：1-2
- 页码：222-227
- 全文大小：169 K
文摘
The impact of HIV-1 genetic diversity on the performance of laboratory testing is an issue that has to be monitored continuously. An “in-house” real-time PCR assay was developed by the Agence Nationale de Recherche sur le SIDA (ANRS) in France for viral load (VL) quantitation based on the amplification of the HIV-1 long terminal repeat (LTR) region. This technology has not been used in Argentina yet and considering the HIV-1 diversity in the country, a comparative analysis of this assay was undertaken versus the Versant HIV-1 RNA 3.0 Assay (b-DNA). The performance was assessed on 30 drug-naïve HIV-1 infected patients who were characterized previously by phylogenetic analysis of the pol and vpu gene. The results showed that there is a significant linear correlation between values of transformed viral load logarithms measured by both, bDNA and real-time PCR assay and that this assay can be used to quantify viral load in samples from BF-infected patients with the same accuracy and reliability as for B subtype samples. The use of “in-house” real-time PCR to measure DNA in PBMCs correlated strongly with the HIV-1 RNA levels in all specimens.