An assay for determining minimal concentrations of antibiotics that drive horizontal transfer of resistance
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- 作者：Jekaterina Jutkina ; Carolin Rutgersson ; Carl-Fredrik Flach ; D.G. Joakim Larsson ; joakim.larsson@fysiologi.gu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Conjugation ; Tetracycline ; Antibiotic resistance ; Mobile genetic elements ; Sub-inhibitory concentration
- 刊名：Science of the Total Environment
- 出版年：2016
- 出版时间：1 April 2016
- 年：2016
- 卷：548-549
- 期：Complete
- 页码：131-138
- 全文大小：732 K
文摘
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Minimal concentrations of antibiotics driving transfer of resistance are not known.
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Previous assays do not separate effects on increased transfer from clonal growth.
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Choice of selective agent, medium and reduced conjugation time improved the assay.
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10 μg/L of tetracycline stimulated horizontal transfer of antibiotic resistance.