Immunophenotyping with CD135 and CD117 predicts the FLT3, IL-7R and TLX3 gene mutations in childhood T-cell acute leukemia

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• 作者：Elda Pereira Noronha ; Francianne Gomes Andrade ; Carolina Zampier ; Camilla F.C.G. de Andrade ; Eugê ; nia Terra-Granado ; Maria S. Pombo-de-Oliveira ; ¹ ; ^{mpombo@inca.gov.br" class="auth_mail" title="E-mail the corresponding author} ; Brazilian Study Group for Childhood Leukaemia BSGCL
• 关键词：ALL ; acute lymphoblastic leukemia ; APC ; allophycocyanin ; AML ; Acute myeloid leukemia ; Bcp-ALL ; B-cell precursor ALL ; BM ; bone marrow ; BFM ; Berlin&ndash ; Frankfurt&ndash ; Munster ; cy ; cytoplasmatic ; DS ; diagnosis sample ; EGIL ; European group for the immunological characterization of leukemia ; FITC ; fluroscein isothiocyanate ; GBTLI-ALL ; Brazilian Group for Treatment of Childhood Leukemia ; ITD ; internal tandem duplication ; MFI ; median fluorescence intensity ; M-FCM ; multiparametric flow cytometry ; MoA
• 刊名：Blood Cells, Molecules and Diseases
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：57
• 期：Complete
• 页码：74-80
• 全文大小：702 K

文摘

With the combination of immunophenotyping and molecular tests, it is still a challenge to identify the characteristics of T cell acute lymphoblastic leukemia (T-ALL) associated with distinct outcomes. This study tests the possible correlation of cellular expression of CD135 and CD117 with somatic gene mutations in T-ALL. One hundred sixty-two samples were tested, including 143 at diagnosis, 15 from T-lymphoblastic lymphoma at relapse, and four relapse samples from sequential follow-up of T-ALL. CD135 and CD117 monoclonal antibodies were included in the T-ALL panel of flow cytometry. The percentage of cells positivity and the median fluorescence intensity were correlated with gene mutational status. STIL-TAL1, TLX3, FLT3 and IL7R mutations were tested using standard techniques.

STIL-TAL1 was found in 24.8%, TLX3 in 12%, IL7R in 10% and FLT3-ITD in 5% of cases. FLT3 and IL7R mutations were mutually exclusive, as were FLT3-ITD and STIL-TAL1. Associations of CD135^high (p < 0.01), CD117^{intermediate/high} (p = 0.02) and FLT3-ITD, CD117^low with IL7R^mutated (p < 0.01) and CD135^high with TLX3^pos were observed.

We conclude that the addition of CD135 and CD117 to the diagnosis can predict molecular aberrations in T-ALL settings, mainly segregating patients with FLT3-ITD, who would benefit from treatment with inhibitors of tyrosine.