Postnatal expression of V₂ vasopressin receptor splice variants in the rat cerebellum

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• 作者：Karina J. Vargas ; Jos&#xe9 ; M. Sarmiento ; Pamela Ehrenfeld ; Carolina C. Añ ; azco ; Carolina I. Villanueva ; Pamela L. Carmona ; Marianne Brenet ; Javier Navarro ; Werner Mü ; ller-Esterl ; Carlos B. Gonz&#xe1 ; lez
• 关键词：Arginine vasopressin ; Vasopressin receptor ; Cerebellar ontogeny ; Purkinje cells ; Motion perception
• 刊名：Differentiation
• 出版年：2009
• 出版时间：April 2009
• 年：2009
• 卷：77
• 期：4
• 页码：377-385
• 全文大小：1600 K

文摘

The V₂ vasopressin receptor gene contains an alternative splice site in exon-3, which leads to the generation of two splice variants (V_2a and V_2b) first identified in the kidney. The open reading frame of the alternatively spliced V_2b transcript encodes a truncated receptor, showing the same amino acid sequence as the canonical V_2a receptor up to the sixth transmembrane segment, but displaying a distinct sequence to the corresponding seventh transmembrane segment and C-terminal domain relative to the V_2a receptor. Here, we demonstrate the postnatal expression of V_2a and V_2b variants in the rat cerebellum. Most importantly, we showed by in situ hybridization and immunocytochemistry that both V₂ splice variants were preferentially expressed in Purkinje cells, from early to late postnatal development. In addition, both variants were transiently expressed in the neuroblastic external granule cells and Bergmann fibers. These results indicate that the cellular distributions of both splice variants are developmentally regulated, and suggest that the transient expression of the V₂ receptor is involved in the mechanisms of cerebellar cytodifferentiation by AVP. Finally, transfected CHO-K1 expressing similar amounts of both V₂ splice variants, as that found in the cerebellum, showed a significant reduction in the surface expression of V_2a receptors, suggesting that the differential expression of the V₂ splice variants regulates the vasopressin signaling in the cerebellum.