Occurrence of a conserved domain in ATP diphosphohydrolases from pathogenic organisms associated to antigenicity in human parasitic diseases

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• 作者：Ana Carolina Ribeiro Gomes Maia^a ; ^b ; Michelle Lima Detoni^a ; ^b ; Gabriane Nascimento Porcino^a ; ^b ; Thais Vieira Soares^a ; ^c ; Michelia Antô ; nia do Nascimento Gusm&#xe3 ; o^a ; ^b ; Melissa Regina Fessel^a ; Marcos Jos&#xe9 ; Marques^d ; Maria Aparecida Souza^e ; Paulo Marcos Zech Coelho^f ; Juliana Assis Silva Gomes Estanislau^g ; Manoel Ot&#xe1 ; vio da Costa Rocha^h ; Marcelo de Oliveira Santos^b ; Priscila Faria-Pinto^a ; ^b ; ^c ; Eveline Gomes Vasconcelos^a ; ^b ; ^c ; ^{eveline.vasconcelos@ufjf.edu.br"" rel=""nofollow}
• 关键词：Potato ; Schistosoma mansoni ; Leishmania (V.) braziliensis ; Trypanosoma cruzi ; Apyrase ; NTPDase ; Polypeptide
• 刊名：Developmental and Comparative Immunology
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：35
• 期：10
• 页码：1059-1067
• 全文大小：623 K

文摘

A polypeptide (r78–117) belonging to the potato apyrase was identified as a conserved domain shared with apyrase-like proteins from distinct pathogenic organisms, and was obtained as a 6xHis tag polypeptide (r-Domain B). By ELISA, high IgG, and IgG1 and IgG2a subtypes levels were detected in BALB/c mice pre-inoculated with r-Domain B. In Schistosoma mansoni adult worm or Leishmania (V.) braziliensis promastigote preparation, anti-r-Domain B antibodies inhibit 22–72 % of the phosphohydrolytic activities and when immobilized on Protein A-Sepharose immunoprecipitate 42–91 % of them. Western blots of the immunoprecipitated resin–antibody–antigen complexes identified bands of mw similar to those predicted for parasite proteins. Total IgG and subclasses of patients with leishmaniasis or schistosomiasis exhibited cross-immunoreactivity with r-Domain B. Therefore, the domain B within both S. mansoni SmATPDase 2 (r156–195) and L. (V.) braziliensis NDPase (r83–122) are potentially involved in the host immune response, and also seem to be conserved during host and parasites co-evolution.