TaqMan RT-PCR and VERSANT^® HIV-1 RNA 3.0 (bDNA) assay: Quantification of HIV-1 RNA viral load in breast milk

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• 作者：Kiersten Israel-Ballard ; Rainer Ziermann ; Christian Leutenegger ; James Di Canzio ; Kimmy Leung ; Lynn Strom ; Barbara Abrams and Caroline Chantry
• 关键词：Breast milk viral load ; Mother-to-child transmission ; bDNA ; TaqMan ; HIV
• 刊名：Journal of Clinical Virology
• 出版年：2005
• 出版时间：December 2005
• 年：2005
• 卷：34
• 期：4
• 页码：253-256
• 全文大小：168 K

文摘

Background: Transmission of HIV via breast milk is a primary cause of pediatric HIV infection in developing countries. Reliable methods to detect breast milk viral load are important.

Objective: To correlate the ability of the VERSANT HIV 3.0 (bDNA) assay to real-time (RT) TaqMan PCR in quantifying breast milk HIV-1 RNA.

Study design: Forty-six breast milk samples that had been spiked with cell-free HIV-1 and eight samples spiked with cell-associated HIV-1 were assayed for HIV-1 RNA by both VERSANT HIV 3.0 and TaqMan RNA assays.

Results: Only assays on the cell-free samples were statistically compared. Both a Deming regression slope and a Bland–Altman slope indicated a linear relationship between the two assays. TaqMan quantitations were on average 2.6 times higher than those of HIV 3.0. A linear relationship was observed between serial dilutions of spiked cell-free HIV-1 and both the VERSANT HIV 3.0 and the TaqMan RNA assays.

Conclusion: The two methods correlated well although the VERSANT HIV 3.0 research protocol quantified HIV-1 RNA slightly lower than TaqMan.