A cell wall protein-based vaccine candidate induce protective immune response against Sporothrix schenckii infection
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- 作者：Deivys Leandro Portuondoa ; deivysleandro@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Alexander Batista-Duharteb ; batistaduhartea@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Lucas Souza Ferreiraa ; gigabreath@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Damiana Té ; llez Martí ; neza ; damianatellezm@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Marisa Campos Polesia ; marisapolesi@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Roberta Aparecida Duartea ; robduarte@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Ana Carolina Alves de Paula e Silvaa ; ana_alpasi@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Caroline Maria Marcosa ; marcos_caroline@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author ; Ana Marisa Fusco de Almeidaa ; almeidaf@fcfar.unesp.br" class="auth_mail" title="E-mail the corresponding author ; Iracilda Zeppone Carlosa ; deivysleandro@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：ssCWP ; S. schenckii cell wall protein ; AH ; aluminum hydroxide ; gp70 ; glycoprotein of 70 ; kD ; PMSF ; phenylmethyl sulfonyl fluoride ; EDTA ; Ethylenediamine tetraacetic acid ; BCA ; bicinchoninic acid ; DTT ; dithiothreitol ; FA ; formic acid ; ACN ; acetonitrile ; FBS ; fetal bovine serum ; NIS ; serum from non-immunized mice ; IS ; serum from S. schenckii-infected mice ; MFI ; median fluorescence intensity ; ADCC ; antibody-dependent cellular cytotoxicity
- 刊名：Immunobiology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：221
- 期：2
- 页码：300-309
- 全文大小：1627 K
文摘
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The DTT-extracted proteins were mainly from Sporothrix schenckii’s cell wall.
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Adsorption of antigens on AH influenced in the profile of immune response.
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Vaccine formulation increased the ex vivo release of IL-12, IFN-γ, IL-4, and IL-17.
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Immune sera showed phagocytosis-enhancing and adhesion-inhibiting properties.
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Passive transference of immune serum protected against S. schenckii infection.