Highly efficient strategy for the heterologous expression and purification of soluble Cowpea chlorotic mottle virus capsid protein and in vitro pH-dependent assembly of virus-like particles
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- 作者：Armando Dí ; az-Vallea ; Yardena M. Garcí ; a-Salcedoa ; Gabriela Chá ; vez-Calvillob ; Laura Silva-Rosalesb ; Mauricio Carrillo-Trippa ; trippm@langebio.cinvestav.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：Capsid protein ; Protein expression purification ; In vitro virus assembly ; Virus like particles ; Cowpea chlorotic mottle virus (CCMV)
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：225
- 期：Complete
- 页码：23-29
- 全文大小：1510 K
文摘
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We present a novel optimized strategy for virus-like particle production.
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We obtain high yields and purity levels of a soluble plant virus capsid protein.
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CPs are in functionally active state with the capacity to readily form VLPs in vitro.
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Our method is efficient and inexpensive, enabling fast implementation.
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Genetic modifications on the CP sequence can be made.