EDG3 and SHC3 on chromosome 9q22 are co-amplified in human ependymomas

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• 作者：Lorenzo Magrassi ; Nicola Marziliano ; Frediano Inzani ; Pamela Cassini ; Ilaria Chiar ; a ; Miran Skrap ; Stefano Pizzolito ; Cesare Arienta ; Eloisa Arbustini
• 关键词：Ependymoma ; Choroid plexus papilloma ; SHC3 ; EDG3 ; Gene amplification
• 刊名：Cancer Letters
• 出版年：2010
• 出版时间：1 April 2010
• 年：2010
• 卷：290
• 期：1
• 页码：36-42
• 全文大小：437 K

文摘

By qPCR we found that EDG3 and SHC3 were amplified in 60 % of ependymomas but none in choroid plexus papillomas. In ependymomas EDG3 and SHC3 amplification increased Shc3 protein levels while EDG3 was less affected. Both proteins were co-immunoprecipitated from ependymoma and Shc3 was tyrosine phosphorylated thus presumably active. We showed by digestion with N-glycosidase-F that EDG3 was glycosylated indicating that EDG3 protein was not retained in the endoplasmic reticulum. The co-immunoprecipitation of Shc3 and EDG3 proteins from ependymomas with amplification of SHC3 and EDG3 genes suggests that the two proteins co-operate and are important for ependymomas in vivo.