Osmotic Regulation of ATA2 mRNA Expression and Amino Acid Transport System A Activity

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• 作者：Alfieri ; Roberta R. ; Petronini ; Pier-Giorgio ; Bonelli ; Mara A. ; Caccamo ; Alessandro E. ; Cavazzoni ; Andrea ; et. al.
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2001
• 出版时间：April 27, 2001
• 年：2001
• 卷：283
• 期：1
• 页码：174-178
• 全文大小：164 K

文摘

When porcine endothelial cells were exposed to hypertonicity, both the level of ATA2 (amino acid transporter 2) mRNA and activity of amino acid transport System A increased transiently, peaking after about 6 and 9 h, respectively. Cycloheximide, like actinomycin D, prevented both responses, showing that an earlier step also involves protein synthesis. Withdrawal of hypertonicity after 6 h increased the rate of down regulation. These findings confirm that ATA2 is a major isoform of System A and show that changes in the expression of ATA2 mRNA precede both the induction and subsequent down regulation of transport activity.