Evaluation of library preparation methods for Illumina next generation sequencing of small amounts of DNA from foodborne parasites
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- 作者：Fernanda S. Nascimentoa ; yeb7@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Yuping Wei-Pridgeona ; b ; saaspservices@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Michael J. Arrowoodc ; mja0@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Delynn Mossc ; dmm3@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Alexandre J. da Silvaa ; 1 ; Alexandre.DaSilva@fda.hhs.gov" class="auth_mail" title="E-mail the corresponding author ; Eldin Talundzicd ; e ; wrj5@cdc.gov" class="auth_mail" title="E-mail the corresponding author ; Yvonne Qvarnstroma ; bvp2@cdc.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Next generation sequencing ; Illumina ; MiSeq ; Library preparation ; Angiostrongylus cantonensis ; Cyclospora cayetanensis
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：130
- 期：Complete
- 页码：23-26
- 全文大小：805 K
文摘
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Illumina genomic libraries were made using one nanogram or less of DNA.
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Sequencing reads from ultra-low input resulted in quality genome assemblies.
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The Ovation Ultralow method produced the best libraries for Illumina sequencing.