Homeopathic remedy made from Toxoplasma gondii reduces the number of bradyzoites in mice infected by the protozoan

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• 作者：Caroline Felicio Braga-Silva^a ; ^{cfeliciobraga@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Ricardo Nascimento Drozino^a ; Neide Martins Moreira^b ; Marcelo Biondaro Gó ; is^c ; Dé ; bora de Mello Gon&ccedil ; ales Sant&rsquo ; Ana^c ; Ana Lú ; cia Falavigna-Guilherme^a ; Silvana Marques de Araú ; jo^a
• 关键词：Toxoplasmosis ; Ophthalmoscopy ; Ocular tonometry ; Mice ; TGF-β
• 刊名：European Journal of Integrative Medicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：7
• 期：5
• 页码：517-524
• 全文大小：441 K

文摘

The search for an effective remedy, with no side effects and with a low cost, is important both to prevent or reduce the damage in toxoplasmosis and to decrease economic losses. This work aims to compare the effect of different dynamizations of Toxoplasma gondii remedy made from the cysts of the parasite.

Methods

In a blind, controlled, randomized assay, 56 Swiss male mice, 60 days old, were divided into groups: BIOT-TG7, BIOT-TG17, BIOT – TG30, BIOT-TG60, BIOT-TG100, BIOT-TG200, GCInf – infected control group pretreated with 7% grain alcohol and GCN – uninfected and untreated control group. Medicines were prepared according to Brazilian Homeopathic Pharmacopoeia, with infected mice's brain macerated (20 T. gondii cysts/100 μL), in the following dynamizations D7, D17, D30, D60, D100 and D200. Pretreatment: 3 consecutive days before inoculation. Infection: At 60 days old, with 20 cysts ME49 strain-T. gondii, orally. Fundoscopy, ocular tonometry, Brain cysts counting, estimated number of bradyzoites/cyst and serum TGF-β (ELISA) were performed on the 60th day post-infection. Statistical comparison: Kruskal–Wallis test, 5% significance level.

Results

Smaller number of cysts (BIOT-TG17 and BIOT-TG200) and a smaller number of bradyzoites/cysts (p < 0.05), in BIOT-TG7, BIOT-TG100 and BIOT-TG200 was observed. 80% of animals from BIOT-TG100 group showed no alterations in fundoscopy and 20% presented subretinal mild hemorrhage around the optic nerve. There was no difference between groups in ocular tonometry and levels of TGF-β.

Conclusion

High dynamizations showed better results in mice infected with T. gondii different from the low dynamizations that had unsatisfactory results.