Characterization of a recombinant multifunctional glycoside hydrolase family 3 β-xylosidase/α-l-arabinofuranosidase/β-glucosidase from Cellulosimicrobium cellulans sp. 21
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- 作者：Ye Yuana ; Yanbo Hua ; Han Zhanga ; Jiayi Lenga ; Fan Lia ; Xuesong Zhaoc ; Juan Gaoa ; b ; gaoj199@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yifa Zhoua ; zhouyf383@nenu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cellulosimicrobium cellulans ; Cloning and expression ; Glycoside hydrolase family 3 ; Substrate specificity ; β-xylosidase/α-l-arabinofuranosidase/β-glucosidase
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：131
- 期：Complete
- 页码：65-72
- 全文大小：3477 K
文摘
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A new GH3 gene ccxyl3a was cloned from C. cellulans sp. 21 and heterologously expressed in E. coli.
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Enzyme CcXyl3A was a multifunctional enzyme with β-xylosidase/α-l-arabinofuranosidase/β-glucosidase activities.
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CcXyl3A synergistically acted with Thermomyces lanuginosus xylanase in beechwood xylan degradation.