High-throughput flow cytometry screening of human hepatocellular carcinoma reveals CD146 to be a novel marker of tumor-initiating cells
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- 作者：Kui Chena ; Avrilynn Dinga ; Yu Dinga ; Anand Ghanekara ; b ; anand.ghanekar@uhn.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cancer ; Hepatocellular carcinoma ; Tumor-initiating cell ; Stem cell ; Flow cytometry ; CD146
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：8
- 期：Complete
- 页码：107-113
- 全文大小：2794 K
文摘
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Unbiased screens of human HCC cells for novel TIC markers have not been reported.
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A high-throughput flow cytometry screen of human HCC cells was successfully performed .
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Candidate TIC markers were further evaluated by RT-PCR and functional assays.
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Of candidates tested, only CD146 expression was significantly increased in HCC tissues.
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CD146+ cells had increased colony-forming capacity, consistent with a TIC phenotype.