Otoprotective effects of erythropoietin on Cdh23^erl/erl mice

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• 作者：F. Han^a ; ^b ; H. Yu^b ; T. Zheng^a ; ^b ; X. Ma^a ; ^b ; X. Zhao^a ; P. Li^a ; L. Le^b ; Y. Su^a ; Q.Y. Zheng^a ; ^b ; ^qyz@case.edu
• 关键词：ABR ; auditory-evoked brainstem response ; DPOAE ; distortion product oto-acoustic emission ; EPO ; erythropoietin ; OHC ; outer hair cell ; PBS ; phosphate-buffered saline ; SPL ; sound pressure level
• 刊名：Neuroscience
• 出版年：2013
• 出版时间：1 May, 2013
• 年：2013
• 卷：237
• 期：Complete
• 页码：1-6
• 全文大小：510 K

文摘

The Cdh23^erl/erl mice are a novel mouse model for DFNB12 and are characterized by progressive hearing loss. In this study, erythropoietin (EPO) was given to the Cdh23^erl/erl mice by intraperitoneal injection every other day from P7 for 7 weeks. Phosphate-buffered saline-treated or untreated Cdh23^erl/erl mice were used as controls. Auditory-evoked brainstem response (ABR) thresholds and distortion product oto-acoustic emission (DPOAE) were measured in the mouse groups at the age of 4, 6 and 8 weeks. The results show that EPO can significantly decrease the ABR thresholds in the Cdh23^erl/erl mice as compared with those of the untreated mice at stimulus frequencies of click, 8-, 16- and 32-kHz at three time points. Meanwhile, DPOAE amplitudes in the EPO-treated Cdh23^erl/erl mouse group were significantly higher than those of the untreated groups at f2 frequency of 15383 Hz at the three time points. Furthermore, the mean percentage of outer hair cell loss at middle through basal turns of cochleae was significantly lower in EPO-treated Cdh23^erl/erl mice than in the untreated mice (P < 0.05). This is the first report that EPO acts as an otoprotectant in a DFNB12 mouse model with progressive hearing loss.