3′,5′-Cyclic diguanylic acid elicits mucosal immunity against bacterial infection

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• 作者：Hongbin Yan ; Rhonda KuoLee ; Kha Tram ; Hongyu Qiu ; Jianbing Zhang ; Girishch ; ra B. Patel ; Wangxue Chen
• 关键词：cdiGMP ; Mucosal adjuvant ; Immunostimulation ; Immunization ; Antibody
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：25 September 2009
• 年：2009
• 卷：387
• 期：3
• 页码：581-584
• 全文大小：271 K

文摘

3′,5′-Cyclic diguanylic acid (cdiGMP) is emerging as a universal bacterial second messenger in regulating bacterial growth on surfaces. It has been recently shown that cdiGMP stimulates innate immunity and enhances antigen-specific humoral and cellular immune responses. We herein report that intranasal (i.n.) administration with cdiGMP induces an acute but transient inflammatory response and activation of dendritic cells in the lungs. Moreover, i.n. immunization of mice with pneumococcal surface adhesion A (PsaA) in conjunction with cdiGMP elicited strong antigen-specific serum immunoglobulin G (IgG) and secretory IgA antibody responses at multiple mucosal surfaces. More importantly, the immunized mice showed significantly reduced nasopharyngeal Streptococcus pneumoniae colonization. These results, for the first time, provide direct evidence for the induction of protection against mucosal bacterial infections by cdiGMP as an adjuvant.