Investigating interactions between phospholipase B-Like 2 and antibodies during Protein A chromatography
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- 作者：Benjamin Trana ; b ; tran.benjamin@gene.com" class="auth_mail" title="E-mail the corresponding author ; Vanessa Grosskopfb ; Xiangdan Wangc ; Jihong Yangc ; Don Walker Jrb ; Christopher Yub ; Paul McDonaldb
- 关键词：Phospholipase B-Like 2 (PLBL2) ; Therapeutic antibody ; Host cell proteins ; Protein A chromatography ; Chinese hamster ovary cell proteins (CHOP)
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：18 March 2016
- 年：2016
- 卷：1438
- 期：Complete
- 页码：31-38
- 全文大小：1489 K
文摘
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PLBL2 levels in the Protein A pool do not always correlate with total host-cell protein levels.
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PLBL2 co-elution during Protein A is highly dependent on antibody titer and PLBL2 titer.
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Antibody load density and intermediate wash conditions can modulate PLBL2 levels in the Protein-A pool.
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PLBL2 interacts preferentially with IgG4 compared to IgG1 subclass antibodies.
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Interaction of PLBL2 ant antibodies is multivalent and likely located in the hinge and/or the CDRs.