Unraveling the secretome of Termitomyces clypeatus grown on agroresidues as a potential source for bioethanol production
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- 作者：Soumya Mukherjee ; Suman Khowala ; sumankhowala@gmail.com" class="auth_mail" title="E-mail the corresponding author ; sumankhowala@yahoo.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：TKP ; tamarind kernel powder ; 2DG ; 2deoxyglucose ; HPLC ; high performance liquid chromatography ; GC ; gas chromatography ; 2D-PAGE ; second dimension polyacrylamide gel electrophoresis ; EG ; endoglucanse ; CBH ; cellobiohydrolase ; PNP ; p-nitrophenol ; PNPX ; p-nitrophenyl-β-d-xylopyranoside ; PNPC ; p-nitrophenyl-β-d-cellobioside ; PNPAF ; p-nitrophenyl-α-l-arabionfuranoside ; PNPA ; p-nitrophenyl acetate ; HPLC ; high performance liquid chromatography ; CAZY ; carbohydrate-active enzymes ; YPD ; yeast extract pepton
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：51
- 期：11
- 页码：1793-1807
- 全文大小：3032 K
文摘
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T. clypeatus produced high titers of lignocellulolytic enzymes in optimized TKP medium.
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Secreted proteins were produced most efficiently at low specific growth rates.
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Majority of the secretome belonged to CAZYmes.
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Synergistic interaction among the cellulases and xylanases improved hydrolysis rate.
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The concentrated hydrolysate produced ∼7.2 g/L ethanol after 6 h fermentation.