A mutant β-glucosidase increases the rate of the cellulose enzymatic hydrolysis
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- 作者：Fá ; bio K. Tamaki1 ; 2 ; É ; verton M. Araujo1 ; Roberto Rozenberg1 ; Sandro R. Marana ; srmarana@iq.usp.br" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sfβgly ; β-glucosidase GH1 from the fall armyworm Spodoptera frugiperda ; TrCel7A ; cellobiohydrolase I from T. reesei ; NPβfuc ; p-nitrophenyl β-fucoside ; NPβgal ; p-nitrophenyl β-galactoside ; NPβglc ; p-nitrophenyl β-glucoside
- 刊名：Biochemistry and Biophysics Reports
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：7
- 期：Complete
- 页码：52-55
- 全文大小：549 K
文摘
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Mutation L428V increased the kcat for cellobiose of the β-glucosidase Sfβgly.
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Sfβgly L428V enhanced the cellobiohydrolase TrCel7A activity upon cellulose.
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Sfβgly L428V raised in 5 fold the initial rate of the cellulose hydrolysis.
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Sfβgly L428V reduced to 1/4 the time needed for TrCel7A run out the substrate.