N-Linked glycans are an important component of the processive machinery of cellobiohydrolases

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• 作者：Alexander V. Gusakov^a ; ^b ; ^{avgusakov@enzyme.chem.msu.ru} ; Anna S. Dotsenko^a ; ^b ; Aleksandra M. Rozhkova^a ; ^b ; Arkady P. Sinitsyn^a ; ^b
• 关键词：Cellobiohydrolase ; Cloning ; N-glycosylation ; Processivity ; Site-directed mutagenesis
• 刊名：Biochimie
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：132
• 期：Complete
• 页码：102-108
• 全文大小：1003 K
• 卷排序：132

文摘

Penicillium verruculosum CBH II (PvCel6A) is glycosylated at four Asn residues. Effects of N-glycans on the activity and processivity of rPvCel6A were studied. N-glycans in PvCel6A affect the pointed parameters either negatively or positively. Mechanism of N-glycan influence seems to be universal for GH6 and GH7 family CBHs. The observed effects are explained in terms of a mechanistic model.