Heterologous expression in Pichia pastoris and characterization of a β-glucosidase from the xylophagous cockroach Panesthia angustipennis spadica displaying high specific activity for cellobiose

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• 作者：Yihai Li^a ; Gaku Arakawa^b ; ^c ; Gaku Tokuda^c ; Hirofumi Watanabe^b ; ^d ; Manabu Arioka^a ; ^{arioka@mail.ecc.u-tokyo.ac.jp}
• 关键词：aa ; amino acids ; CBB ; Coomassie Brilliant Blue ; CBH ; cellobiohydrolase ; EG ; endoglucanase ; Endo H ; endoglycosidase H ; GPF ; glycopeptidase F ; pNP ; p-nitrophenol ; pNPG ; p-nitrophenyl β-d-glucopyranoside ; TLC ; thin layer chromatography
• 刊名：Enzyme and Microbial Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：97
• 期：Complete
• 页码：104-113
• 全文大小：1586 K
• 卷排序：97

文摘

A β-glucosidase, PaBG1b, from the xylophagous cockroach Panesthia angustipennis spadica was heterologously expressed and characterized. PaBG1b demonstrated high specific activity and catalytic efficiency towards cellobiose with Vmax and kcat/Km values of 436.7 ± 6.3 U/mg and 109.8 mM−1 s−1, respectively. Although the glucose tolerance of PaBG1b was moderate (Ki = 200.3 ± 1.1 mM), it was not inhibited by cellobiose up to the highest concentration tested (100 mM), which makes it a suitable candidate for commercial bioethanol production from cellulose.