Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator
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- 作者：Xiaoyue Zhanga ; Yonghao Lia ; Xinqing Zhaob ; xqzhao@sjtu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Fengwu Baia ; b
- 关键词：Trichoderma reesei ; Cellulase production ; Glucose ; xyr1 and cre1 ; Artificial transcription factor
- 刊名：Bioresource Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：223
- 期：Complete
- 页码：317-322
- 全文大小：752 K
文摘
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An artificial transcription activator xyr1-cre1b was expressed in T. reesei Rut C30.
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The mutants displayed constitutive (hemi) cellulase production from glucose.
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FPase of 2.63 IU/mL was achieved within short time (48 h) in a 7-L fermenter.
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The crude enzyme is suitable for sugar release from alkali pretreated corn stover.