Role of E-cadherin N

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• 作者：Salomé ; S. Pinho ; Hugo Osó ; rio ; Mihai Nita-Lazar ; Joana Gomes ; Cé ; lia Lopes ; Fá ; tima Gä ; rtner ; Celso A. Reis
• 关键词：E-cadherin ; N-glycosylation ; Mammary gland tumor ; Adenoma ; Carcinoma ; Canine
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：20 February 2009
• 年：2009
• 卷：379
• 期：4
• 页码：1091-1096
• 全文大小：3202 K

文摘

Modifications in cell surface glycosylation affecting cell adhesion are common characteristics of transformed cells. This study characterizes the N-glycosylation profile of E-cadherin in models of canine mammary gland adenoma and carcinoma evaluating the importance of these glycosylation modifications in the malignant phenotype.

Our results show that the pattern of E-cadherin N-glycosylation in mammary carcinoma is characterized by highly branched N-glycans, increase in sialylation and an expression of few high mannose structures. Detailed mass spectrometry analysis demonstrated a new N-glycosylation site containing a potential complex type N-glycan in E-cadherin from a mammary carcinoma cell line.

Our study demonstrates the importance of E-cadherin N-glycans in the process of tumor development and in the transformation to the malignant phenotype.