Assay to measure sphingomyelinase and ceramidase activities efficiently and safely
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- 作者：Christiane Mü ; hle ; christiane.muehle@uk-erlangen.de ; christiane.muehle@molbio-research.eu ; Johannes Kornhuber johannes.kornhuber@uk-erlangen.de
- 关键词：Ceramidase ; Enzyme activity assay ; Fluorescent substrate ; Sphingomyelinase ; Thin layer chromatography ; High throughput
- 刊名：Journal of Chromatography A
- 出版年：2017
- 出版时间：20 January 2017
- 年：2017
- 卷：1481
- 期：Complete
- 页码：137-144
- 全文大小：1514 K
- 卷排序：1481
文摘
Fluorescent sphingomyelin is a safe, low-cost alternative to radioactive substrate. Ethylacetate/acetic acid can replace toxic solvents for both enzyme activity assays. Chromatography of reaction mixtures can be performed without prior extraction. Different plasma sources and serum yield equivalent sphingomyelinase activities.