Response of plasma copper, ceruloplasmin, iron and ions in carp, Cyprinus carpio to waterborne copper ion and nanoparticle exposure

详细信息    查看全文

• 作者：Aliakbar Hedayati ; Seyyed Morteza Hoseini ; ^{seyyedmorteza.hoseini@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Seyed Hossein Hoseinifar
• 关键词：Copper ; Nanoparticle ; Fish ; Physiology ; Iron
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：87-93
• 全文大小：687 K

文摘

In this study, Cyprinus carpio was exposed to 0.25 mg L^− 1 copper as either copper sulfate (CuSO₄) or copper oxide nanoparticles (nano-Cu), and 25 mg L^− 1 copper as nano-Cu for 14 days. CuSO₄ and high concentration of nano-Cu led to a significant increase in plasma total copper levels. Plasma free copper levels increased significantly in all copper-exposed treatments. Except for low concentration of nano-Cu after 7 and 14 days, copper exposure generally led to a significant decrease in plasma ceruloplasmin levels. Plasma iron levels increased significantly in CuSO₄ (all times) and nano-Cu (7th and 14th days) treatments. A significant elevation in plasma total iron binding capacity (TIBC) was observed after 3 days in the fish exposed to low concentration nano-Cu, and after 14 days in all copper-exposed treatments. Transferrin saturation (TSA) ratio showed significant increase in CuSO₄ (3rd and 7th days) and the high concentration nano-Cu (7th day) treatments. Decrease in plasma chloride (7th and 14th days) and sodium (14th days) was observed in CuSO₄ treatment. In conclusion, the results suggest that ionic copper is mainly absorbed via fish gill, whereas, nano-copper are more likely absorbed via gut. Also, data suggest that ionic copper has more adverse effects on the examined plasma biochemical characteristics compared to the equivalent nano-copper concentration, which may be due to the lower copper absorption by fish in the nano-copper suspension.