Prediction of gradient retention data for hydrophilic interaction liquid chromatographic separation of native and fluorescently labeled oligosaccharides

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• 作者：Nikola Vaňková ; Petr Česla ; ^{Petr.Cesla@upce.cz}
• 关键词：Hydrophilic interaction liquid chromatography ; Gradient elution ; Prediction ; Oligomers ; Mixed-mode retention model
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：17 February 2017
• 年：2017
• 卷：1485
• 期：Complete
• 页码：82-89
• 全文大小：2184 K
• 卷排序：1485

文摘

Gradient retention times of maltooligosaccharides were predicted. Prediction accuracy was compared for isocratic and gradient input data. 15 gradient profiles with different steepnesses were compared. Four types of derivatization agents were studied. Method development was speeded-up using oligomeric mixed-mode model.