Rose Bengal and Lissamine Green Inhibit Detection of Herpes Simplex Virus by PCR

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• 作者：Gerami D. Seitzman ; Vicky Cevallos and Todd P. Margolis
• 刊名：American Journal of Ophthalmology
• 出版年：2006
• 出版时间：April 2006
• 年：2006
• 卷：141
• 期：4
• 页码：756-758
• 全文大小：130 K

文摘

Purpose

To determine whether rose bengal and lissamine green affect polymerase chain reaction (PCR) detection of herpes simplex virus (HSV).

Design

Laboratory investigation.

Methods

Diagnostic corneal scrapings were evaluated for PCR inhibitory activity. Dacron swabs inoculated with rose bengal and lissamine green were processed as clinical samples, inoculated with control HSV, varicella zoster (VZV), cytomegalovirus (CMV), and toxoplasma DNA and prepared for PCR. The effects of calcium alginate and cotton swabs were also evaluated.

Results

Rose bengal, lissamine green, and calcium alginate not only inhibit PCR detection of HSV DNA, but also detection of VZV, CMV, and toxoplasma DNA. This inhibition could be overcome by serial dilution and by DNA purification of the sample before PCR.

Conclusions

Rose bengal, lissamine green, and calcium alginate can inhibit PCR detection of HSV DNA. Clinical scrapings to be sent for PCR diagnostic testing should be taken before instillation of rose bengal or lissamine green.