cGMP and cyclic nucleotide-gated channels participate in mouse sperm capacitation

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• 作者：Abraham Cisneros-Mejorado^a ; Daniel P. Sá ; nchez Herrera^b ; ^{dpaulo@cinvestav.mx}
• 关键词：8Br-cAMP ; 8-bromoadenosine 3&prime ; 5&prime ; -cyclic monophosphate ; 8Br-cGMP ; 8-bromoguanosine 3&prime ; 5&prime ; -cyclic monophosphate ; cAMP ; cyclic adenosine monophosphate ; cGMP ; cyclic guanosine monophosphate ; CNG ; cyclic nucleotide-gated ; HEPES-Na ; 4-(2-
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：20 January 2012
• 年：2012
• 卷：586
• 期：2
• 页码：149-153
• 全文大小：534 K

文摘

During capacitation of mammalian sperm intracellular [Ca²⁺] and cyclic nucleotides increase, suggesting that CNG channels play a role in the physiology of sperm. Here we study the effect of capacitation, 8Br-cAMP (8-bromoadenosine 3?5?cyclic monophosphate) and 8Br-cGMP (8-bromoguanosine 3?5?cyclic monophosphate) on the macroscopic ionic currents of mouse sperm, finding the existence of different populations of sperm, in terms of the recorded current and its response to cyclic nucleotides. Our results show that capacitation and cyclic nucleotides increase the ionic current, having a differential sensitivity to cGMP (cyclic guanosine monophosphate) and cAMP (cyclic adenosine monophosphate). Using a specific inhibitor we determine the contribution of CNG channels to macroscopic current and capacitation.