Detection and quantification of the selective EP4 receptor antagonist CJ-023423 (grapiprant) in canine plasma by HPLC with spectrofluorimetric detection
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- 作者：Virgina De Vitoa ; Alessandro Sabab ; Hong-Ki Leec ; Helen Owend ; Amnart Poapolathepe ; Mario Giorgif ; mario.giorgi@unipi.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：PGE2 ; prostaglandin E2 ; IS ; internal standard ; ACN ; acetonitrile ; MeOH ; methanol ; CH2Cl2 ; dichloromethane ; Et2O ; diethyl ether ; CHCl3 ; chloroform ; C3H8O ; 2-propanol ; AcOEt ; ethyl acetate ; AcONH4 ; ammonium acetate ; HCl ; hydrochloric acid ; NaOH ; sodium hydroxide ; ESI ; electrospray ionization source ; Ps ; positive ion mode ; Ng ; negative ion mode ; LOD ; limit of detection ; LLOQ ; lower limit of quantification ; QC ; quality control ; RE ; relative error ; SD ; standard deviation ; P ; probability level ; RSD ; p
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：25 January 2016
- 年：2016
- 卷：118
- 期：Complete
- 页码：251-258
- 全文大小：590 K
文摘
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Grapiprant is a new active ingredient under development for the control of pain and inflammation.
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A validated HPLC method with spectrofluorimetric detection has been developed.
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The chromatographic runs were specific with no interfering peaks at the retention times of the analyte and IS.
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LOD and LLOQ were 3 and 10 ng/mL.
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This method is suitable for pharmacokinetic investigations such as guiding dose adjustment.