Agrobacterium tumefaciens-mediated transformation of the entomopathogenic fungus Nomuraea rileyi
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- 作者：Changwen Shaoa ; b ; changwen2000@sina.com" class="auth_mail" title="E-mail the corresponding author ; Youping Yina ; ypy128@vip.sina.com" class="auth_mail" title="E-mail the corresponding author ; Zhaoran Qia ; qzr20101901003@163.com" class="auth_mail" title="E-mail the corresponding author ; Ren Lia ; 19881021.ren@163.com" class="auth_mail" title="E-mail the corresponding author ; Zhangyong Songa ; szy83529@163.com" class="auth_mail" title="E-mail the corresponding author ; Yan Lia ; sungq520@yeah.net" class="auth_mail" title="E-mail the corresponding author ; Zhongkang Wanga ; w-zk@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：SMAY ; Sabouraud&rsquo ; s maltose agar yeast medium ; SOE-PCR ; splicing by overlap extension PCR ; AS ; acetosyringone ; IM ; induced medium ; FPNI-PCR ; fusion primer and nested integrated PCR
- 刊名：Fungal Genetics and Biology
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：83
- 期：Complete
- 页码：19-25
- 全文大小：2295 K
文摘
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An Agrobacterium-mediated transformation system for the entomopathogenic fungus N. rileyi was established.
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N. rileyi blastospores have a higher transformation efficiency compared with pre-cultured conidia.
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DsRed2 was used as a visible marker to readily detect transformants by fluorescence microscopy.
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The transformation protocol can be used to genetically manipulate N. rileyi and potentially other similar fungal pathogens.