A photoactivable ZBpa–Biotin was fabricated by aaRS/tRNA and Avitag/BirA techniques.
A approach for Fc-specific photo-biotinylated IgG via ZBpa–Biotin was proposed.
The photo-biotinylated IgG was used to fabricate an immunosensor for detecting CEA.
It gave a LOD of 2 ng mL-1 CEA, was 5-fold lower than that of NHS-biotinylated IgG.