Effects of IKK? on Oxidised Low-density Lipoprotein-induced Injury in Vascular Endothelial Cells

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• 作者：Changchun Cao ; PhD ; MD^b ; ¹ ; Yongchao Qi ; MD^a ; ¹ ; Wen Chen ; MD^a ; Yifan Zhu ; MD^a ; Xin Chen ; PhD ; MD^a ; ^{stevecx@njmu.edu.cn} ; ^{qiyongchao1@126.com}
• 关键词：IKK? ; Vascular endothelial cells ; Oxidised low-density lipoprotein ; NF-¦ÊB
• 刊名：Heart, Lung and Circulation
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：22
• 期：5
• 页码：366-372
• 全文大小：1180 K

文摘

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Background

Oxidised low-density lipoprotein (ox-LDL) led to endothelial dysfunction, which was an initial step in the formation of atheroma. The role of IKK? in Ox-LDL-induced endothelial cell injury was investigated in this study.

Methods

Primary cultures of endothelial cell were identified by the immunofluorescent detection of von Willebrand's factor (vWF). Wild-type and IKK? knockout endothelial cells were treated with ox-LDL and effects on ultrastructural morphology were evaluated by transmission electron microscopy. Western blotting analysis was conducted to evaluate effects on IL-18, VEGF, IKK¦Á, IKK¦Â, IKK?, p65 and p50 expression.

Results

From the ultrastructural morphology of endothelial cells and the expression of inflammatory factor such as IL-18 and VEGF, it was suggested that IKK? knockout endothelial cells showed less severe ox-LDL-induced injury compared to wild-type endothelial cells. Ox-LDL increased IKK? and phosphorylated IKK? expression in wild-type endothelial cells, while no significant change in IKK¦Á and IKK¦Â levels was observed. Ox-LDL significantly increased the expression of p65(or p50) and phosphorylated p65(or p50) in wild-type endothelial cells, while this effect was completely blocked in IKK? knockout endothelial cells.

Conclusions

IKK? might play an important role in ox-LDL-induced injury of endothelial cells.