A pure polysaccharide from Ephedra sinica treating on arthritis and inhibiting cytokines expression

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• 作者：Qiuhong Wang^a ; ^b ; ¹ ; Zunpeng Shu^b ; ¹ ; Na Xing^b ; Bingqing Xu^b ; Changfu Wang^b ; Guibo Sun^a ; Xiaobo Sun^a ; ^{sunsubmit@163.com" class="auth_mail" title="E-mail the corresponding author} ; Haixue Kuang^b ; ^{haixuekuang@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Ephedra sinica Stapf polysaccharide ; Rheumatoid arthritis ; TLR4 ; Signaling pathway
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：86
• 期：Complete
• 页码：177-188
• 全文大小：2777 K

文摘

In our previous study, we found that the acidic polysaccharides of Ephedra sinica had immunosuppressive effect to treat rheumatoid arthritis and the pure polysaccharide ESP-B4 was the main composition of the acidic polysaccharides. At present, the exact molecular mechanism of ESP-B4 on treating arthritis is unclear. We are thus evaluating the properties of ESP-B4 on LPS-induced THP-1 pro-monocytic cells and adjuvant-induced arthritis in Wistar rats via TLR4. In vitro, ESP-B4 decreased the production of cytokines induced by LPS. In addition, ESP-B4 reduced the LPS-stimulated nuclear translocation of p65 subunit of NF-κB. Pretreatment with ESP-B4 significantly down-regulated the phosphorylation of MAPKs induced by LPS. Furthermore, in vivo, after 12 days of disease induced by adjuvant, rats were treated with ESP-B4 for 16 days. ESP-B4 significantly improved all parameters of inflammation. ESP-B4 reduced the release of inflammatory factors and cytokines by inhibiting the TLR4 signaling pathway to treat rheumatoid arthritis.