Development of one-step reverse transcription loop-mediated isothermal amplification for norovirus detection in oysters

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• 作者：Su Been Jeon^a ; Dong Joo Seo^a ; Hyejin Oh^a ; David H. Kingsley^b ; Changsun Choi^a ; ^{cchoi@cau.ac.kr}
• 关键词：Human norovirus ; Loop-mediated isothermal amplification ; Oyster ; Detection ; Foodborne virus
• 刊名：Food Control
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：73
• 期：part_PB
• 页码：1002-1009
• 全文大小：899 K
• 卷排序：73

文摘

One-step RT-LAMP was developed for detecting human norovirus GI and GII. One-step RT-LAMP for NoV was 10–100 times more sensitive than realtime RT-PCR. One-step RT-LAMP detected NoV in oyster hemocytes and oyster tissues.