Suppressive effect of diazepam on IFN-γ production by human T cells

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• 作者：Min Wei ; Li Li ; Rui Meng ; Yanying Fan ; Yun Liu ; Liang Tao ; Xianguo Liu ; Changyou Wu
• 关键词：Diazepam ; Benzodiazepine ; PBRs ; T cells ; Immunity
• 刊名：International Immunopharmacology
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：10
• 期：3
• 页码：267-271
• 全文大小：402 K

文摘

Many studies showed that benzodiazepines could modulate immune responses through interaction with peripheral benzodiazepine receptors (PBRs) in immune cells but most of the studies were focused on monocytes and macrophages. In the present study, we revealed that diazepam, a mixed-type benzodiazepine, inhibited IFN-γ production by human peripheral blood mononuclear cells (PBMCs) induced by anti-CD3 in dose-dependent manner. Flow cytometry analysis demonstrated that diazepam could inhibit the frequency of IFN-γ-producing CD4⁺ and CD8⁺ T cells. The inhibitory effect of diazepam on IFN-γ production is similar to that of R₀5-4864, a selective PBRs ligand. However, D8555, a selective ligand for PBRs in microglia in the central nervous system, is a much weak inhibitor compared with R₀5-4864 or diazepam. The inhibitory effect of R₀5-4864 could be antagonized by PK11195, which is recognized as selective PBRs antagonist, and suppressive effect of diazepam on T cells is partially antagonized by PK11195. Collectively, these results suggested that diazepam suppressed human T cell function through PBRs.