Dioscin alleviates lipopolysaccharide-induced inflammatory kidney injury via the microRNA let-7i/TLR4/MyD88 signaling pathway

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• 作者：Meng Qi ; Lianhong Yin ; Lina Xu ; Xufeng Tao ; Yan Qi ; Xu Han ; Changyuan Wang ; Youwei Xu ; Huijun Sun ; Kexin Liu ; Jinyong Peng ; ^{jinyongpeng2008@126.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：LPS ; lipopolysaccharide ; MDA ; malondialdehyde ; NO ; nitric oxide ; ROS ; reactive oxygen species ; GSH ; Px glutathione peroxidase ; CAT ; catalase ; TLR4 ; toll like receptor 4 ; MyD88 ; myeloid differentiation primary response gene ; NOX1 ; NADPH oxidase 1 ; NF-κB ; nuclear factor kappa B ; PI3K ; phosphatidylinositol 3-kinases ; Akt ; serine/threonine kinase ; SOD2 ; superoxide dismutase ; IL-1β ; interleukin-1β ; IL-6 ; interleukin-6 ; MIP-1α ; macrophage inflammatory protein-1α ; Fas ; factor associated with suicide ; F
• 刊名：Pharmacological Research
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：111
• 期：Complete
• 页码：509-522
• 全文大小：8349 K

文摘

We previously reported the potent effect of dioscin against renal ischemia/reperfusion injury, but little is known about the role of dioscin in lipopolysaccharide (LPS)-induced inflammatory kidney injury. The present work aimed to investigate the effects and potential mechanisms of dioscin in preventing LPS-induced kidney injury. In vivo injury was induced in rats and mice with an intraperitoneal injection of LPS (10 mg/kg), and in vitro studies were performed on NRK-52E and HK-2 cells challenged with LPS (0.5 μg/ml). Our results indicated that dioscin significantly protected against renal damage by decreasing blood urea nitrogen and creatinine levels and reversing oxidative stress. Mechanistic studies demonstrated that dioscin markedly up- regulated the level of the microRNA let-7i, resulting in significant inhibition of TLR4 expression. Dioscin significantly down-regulated the levels of MyD88, NOX1 and cleaved caspase-8/3; inhibited the nuclear translocation of NF-κB; inhibited PI3K and Akt phosphorylation; increased the levels of SOD2; and decreased the mRNA levels of IL-1β, IL-6, MIP-1α, Fas and FasL. In vitro, transfection of microRNA let-7i inhibitor and TLR4 DNA were applied, and the results further confirmed the nephroprotective effect of dioscin in suppressing TLR4/MyD88 signaling and subsequently inhibiting inflammation, oxidative stress and apoptosis. Furthermore, the abrogation of cellular MyD88 expression by ST2825 eliminated the inhibitory effect of dioscin on the levels of nuclear NF-κB, cleaved caspase-3, SOD2 and ROS. These data indicated that dioscin exerted a nephroprotective effect against LPS-induced inflammatory renal injury by adjusting the microRNA let-7i/TLR4/MyD88 signaling pathway, which provided novel insights into the mechanisms of this therapeutic candidate for the treatment of inflammatory kidney injury.