Deciphering flux adjustments of engineered E. coli cells during fermentation with changing growth conditions

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• 作者：Lian He^a ; ¹ ; Yu Xiu^b ; ^c ; ¹ ; J. Andrew Jones^b ; ^d ; ¹ ; Edward E.K. Baidoo^e ; Jay D. Keasling^e ; ^f ; ^g ; ^h ; ⁱ ; ^j ; Yinjie J. Tang^a ; ^{yinjie.tang@wustl.edu} ; Mattheos A.G. Koffas^b ; ^k ; ^{koffam@rpi.edu}
• 关键词：13C-MFA ; Channeling ; Free metabolites ; Promoter ; Reflux ; Tryptophan ; Violacein
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：247-256
• 全文大小：1394 K
• 卷排序：39

文摘

Maintaining metabolic robustness is a primary choice. Adaptability is an alternative but beneficial choice. Extensive differences are seen between high- and low-violacein producing E.coli. Our findings support strategies that split growth and molecule-production phases.