Impedimetric immunosensor for the specific label free detection of ciprofloxacin antibiotic
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- 作者：Rodica E. Ionescu ; Nicole Jaffrezic-Renault ; Laurent Bouffier ; Chantal Gondran ; Serge Cosnier ; Daniel G. Pinacho ; M.-Pilar Marco ; Francisco J. Sá ; nchez-Baeza ; Thomas Healy ; Claude Martelet
- 关键词：Ciprofloxacin ; Immunosensor ; Polypyrrole ; Impedance spectroscopy
- 刊名：Biosensors and Bioelectronics
- 出版年：2007
- 出版时间：30 November 2007
- 年：2007
- 卷：23
- 期：4
- 页码：549-555
- 全文大小：774 K
文摘
Impedance spectroscopy approaches combined with the immunosensor technology have been used for the determination of trace amounts of ciprofloxacin antibiotic belonging to the fluoroquinolone family. The sensor electrode was based on the immobilization of anti-ciprofloxacin antibodies by chemical binding onto a poly(pyrrole-NHS) film electrogenerated on a solid gold substrate. The electrode surface was modified by electropolymerization of pyrrole-NHS, antibody grafting and ciprofloxacin immunoreaction. The sensitive steps of surface modification, cyclic voltammetry (CV) and atomic force microscopy (AFM) imaging have been used for electrode surface characterization.
The immunoreaction of ciprofloxacin on the grafted anti-ciprofloxacin antibody directly triggers a signal via impedance spectroscopy measurements which allows the detection of extremely low concentration of 10 pg/ml ciprofloxacin.