Electropolymerized biotinylated poly (pyrrole–viologen) film as platform for the development of reagentless impedimetric immunosensors

详细信息    查看全文

• 作者：Chantal Gondran ; Maylis Orio ; David Rigal ; Bruno Gall ; Laurent Bouffier ; Tioga Gulon ; Serge Cosnier
• 关键词：Viologen ; Impedance ; Polypyrrole ; Biotin ; Immunosensor
• 刊名：Electrochemistry Communications
• 出版年：2010
• 出版时间：February 2010
• 年：2010
• 卷：12
• 期：2
• 页码：311-314
• 全文大小：223 K

文摘

We describe herein the synthesis and electrochemical polymerization of a viologen monomer functionalized by a biotin group and the use of its redox properties for the impedimetric sensing of protein binding. The electrochemical oxidation of the pyrrole group at 0.85 V in CH₃CN led to a biotinylated polypyrrole film allowing the successive anchoring of an avidin layer and a biotinylated cholera toxin layer. EIS performed at −0.45 V/SCE without redox probe in solution showed the specific immobilization of the anti-cholera toxin antibody.