Molecular cloning, characterization and tissue specificity of the expression of the ovine CSRP2 and CSRP3 genes from Small-tail Han sheep (Ovis aries)
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- 作者：Guanqing Liu ; Chunlan Zhang ; Guizhi Wang ; Zhibin Ji ; Zhaohua Liu ; Tianle Chao ; Saisai Zhang ; Jianmin Wang ; wangjm@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：CSRP2 ; cysteine- and glycine-rich protein 2 ; CSRP3 ; cysteine- and glycine-rich protein 3 ; CRP2 ; cysteine-rich protein 2 ; CRP3 ; cysteine-rich protein 3 ; SH ; Small-tail Han sheep ; DP ; Dorper sheep ; LD ; longissimus dorsi ; BF ; biceps femoris ; GAPDH ; glyceraldehyde-6-phosphate dehydrogenase ; ORF ; open reading frame ; qRT-PCR ; quantitative reverse transcription polymerase chain reaction ; RACE ; rapid amplification of cDNA ends ; SE ; standard error ; TGF ; transforming growth factor ; UTR ; untranslated regio
- 刊名：Gene
- 出版年：2016
- 出版时间：10 April 2016
- 年：2016
- 卷：580
- 期：1
- 页码：47-57
- 全文大小：3148 K
文摘
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We clone and identify CSRP2 and CSRP3 genes from Small-tail Han Sheep.
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CRP2 and CRP3 characterize, structure and phylogenetic analysis are studied.
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mRNA expression of two genes in different tissues in two breed sheep is performed.
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Protein expression of CRP3 in different tissues in two breed sheep is performed.