Fosmid library construction and screening for the maize mutant gene Vestigial glume 1

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• 作者：Chaoxian Liu^a ; ¹ ; Xiaoli Liu^a ; ¹ ; Lei Lei^b ; Haiying Guan^c ; Yilin Cai^a ; ^{caiyilin1789@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Vestigial glume 1 ; Fosmid ; Library construction ; Library screening
• 刊名：The Crop Journal
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：4
• 期：1
• 页码：55-60
• 全文大小：1069 K

文摘

The maize mutant gene Vestigial glume 1 (Vg1) has been fine-mapped to a narrow region by map-based cloning and the candidate gene for Vg1 spanned 19.5 kb. Here we report Vg1 genomic fosmid library construction and screening. The fosmid library of Vg1 consisted of 574,000 clones with an average insert size of 36.4 kb, representing 7.9-fold coverage of the maize genome. Fosmid stability assays indicated that clones were stable during propagation in the fosmid system. Using Vg1 candidate gene-specific primers, a positive clone was successfully identified. This discovery will pave the way for identifying the function of Vg1 in maize development.