Nucleotide-free kinesin motor domains reversibly convert to an inactive conformation with characteristics of a molten globule
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- 作者：David D. Hackney ; ddh@andrew.cmu.edu" class="auth_mail" title="E-mail the corresponding author ; Marshall S. McGoff
- 关键词：Kinesin ; Molten globule ; Conformational selection ; Induced fit ; Osmolyte effect
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：608
- 期：Complete
- 页码：42-51
- 全文大小：1705 K
文摘
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Apo-kinesin reversibly converts to an inactive conformation.
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Inactive conformation has characteristics of a molten globule.
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Glycerol stabilizes active form by selectively reducing the rate of inactivation.
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Reactivation in the presence of ADP occurs by conformational selection.