Tablysin 2 is a fibrinogenolytic enzyme from salivary glands of horsefly.
Tablysin 2 was expressed in E. coli with pET-32a (+) plasmid as fusion protein.
Tablysin 2 is anticoagulant by hydrolyzing fibrinogen and inhibiting platelets.
A method for expression of trace proteins in horsefly salivary gland is presented.